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KMID : 0380019950100010105
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 1 p.105 ~ p.111
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Development of Biomolecular Device Using Biomolecular Film
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Abstract
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The fiber-optic biosensor using enzyme-immobilized Langmuir-Blodgett film is developed for the measurement of ethanol. The enzyme, alcohol dehydrogenase, is immobilized at the molecular level on the arachidic acid monolayer using Langmuir-Blodgett film technique. Based on the ordered multisubstrate mechanism, the immobilized enzyme kinetics is investigated. The optical sensing system is proposed, and sensor signal is proportional to ethanol concentraion and is related with the number of enzyme layers. As the number of deposited LB film layer increases up to 20layers, the high ethanol concentration of 45mM can be measured without the saturation of signal. Surface pressure-area isotherm is measured for the three differnt charged-lipids. Arachidic acid is the most suitable for the adsorption of alcohol dehydrogenase based on electrostatic force.
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